Progress in research of long-term protective efficacy of human papillomavirus vaccine / 中华流行病学杂志

The efficacy of HPV vaccine in preventing cervical cancer has been demonstrated in numerous clinical trials and clinical uses. The follow-up after clinical trials usually last for 5-6 years to evaluate the long-term efficacy, and a series of long-term follow-up studies have been conducted in some regions. The literature retrieval of HPV vaccine long term efficiency research both at home and abroad indicated that the protective efficacy of the vaccine against vaccine-type-related cervical intraepithelial neoplasia grade 2 and above is higher than 90%.

Exploration of the molecular mechanism of Lishi-Kuijie decoction in the treatment of ulcerative colitis based on network pharmacology / 药学学报

We used network pharmacology and molecular docking to investigate the molecular mechanism of Lishi-Kuijie decoction (KJF) in the treatment of ulcerative colitis (UC). Chemical components and targets related to the 13 herbs of Chinese Materia Medical in KJF were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The UC-related targets were identified through OMIM, DisGeNet and GeneCards databases. Using Cytoscape 3.7.2 software a drug-compound-disease-target network was established. The target interaction network and core target for KJF against UC was built and selected based on the String database and topological parameters. Using the R package clusterprofile in Bioconductor, the intersection genes and the disease-drug intersection targets were transformed to Entrez gene ID, followed by gene ontology biological process enrichment analysis and KEGG pathway annotation analysis. The KJF compound-UC target network contained 149 compounds, 108 corresponding targets and 12 core targets (including signal transducer and activator of transcription 3, interleukin 6, tumor necrosis factor, c-x-c motif chemokine ligand 8, interleukin 2, etc.). We identified 2 371 GO terms and 155 pathways (mainly involving IBD, PI3K-ATK, NF-kappa B, TNF, Toll-like receptor signaling pathway) as determined by enrichment analysis. Molecular docking, used with the key molecular factors and the core targets, revealed stable binding for IL2, TNF-α, MAPK1 and RELA. These results suggest the possible molecular mechanism of KJF in treatment of UC and lay the foundation for further characterization of the components and their mechanisms.

Expression of PD1/PD-L1 in gastric carcinoma and its correlation with clinicopathological characteristics and prognosis / 临床与实验病理学杂志

Purpose To investigate the expression of pro-grammed death1 (PD-1), programmed death-ligand1 ( PD-L1) in gastric carcinoma and its correlation with clinicopathological characteristics and prognosis. Method Immunohistochemistry was used to dectect the expression of PD-1/PD-L1 in 75 cases of gastric cancer and adjacent tissues of cancer, without chemother-apy and radiotherapy from 2011 to 2016 in The Affiliated Jiangsu Shengze Hospital of Nanjing Medical University. To analyzed the relationship between expression of PD-1/PD-L1 in gastric carci-noma and clinicopathologic characteristics, and analyzed rela-tionship of overall survival time in all patients. Result The tumor cells expressed 37. 3% (28/75) PD-L1 positive rate in 75 cases, which associated with age, tumor diameter and differ-entiation. PD-1 positive rate in the tumor infiltrating lympho-cytes was 46. 7% (35/75), which associated with pTNM stag-ing. The positively rate of PD-L1 expression in tumor cells and PD-1 expression in the tumor infiltrating lymphocytes were sig-nificantly higher than that in the adjacent tissues of carcinoma (P＜0. 001). Kaplan-Meier and Long-rank results showed the overall survival time was significantly related with tumor diame-ter, differentiation, invasion depth, metastasis and pTNM stag-ing ( P ＜0. 05), but unrelated with PD-1 and PD-L1 expres-sion. But if the follow-up time was 14 months, the prognosis of PD-L1 positive patients were significantly lower than that of the negative ones. Conclusion Expression of PD-1/PD-L1 in cancer cells are significantly higher than that in para-carcinoma tissue. Positive expression of PD-L1 in tumor cells may contrib-ute to evaluation of short-term prognosis, and has nothing to do with the overall survival rate.

Advancement in methods of evaluation on bone fracture healing and its application in forensic medicine / 法医学杂志

It is frequently encountered to identify the time of bone fracture in forensic medicine. Hence it is important to develop the methods for evaluating the time of bone fracture. This article reviews the applications and values of the methods such as ultrasonic evaluation, impulse measurement, digital imaging technology and bone mineral density testing technology, etc. It is proposed that to use these methods jointly may provide more scientific evidence in determine the time of bone fracture.

The PRVEPs evoked by different fields stimulation / 法医学杂志

OBJECTIVE@#To study the character of the PRVEPs evoked by different stimulation fields in normal-vision subjects and its value in forensic medicine.@*METHODS@#The PRVEPs were evoked by full-field, central-field and peripheral-field respectively in 30 subjects (60 eyes) and the latency and amplitude of N1 and P1 were analyzed.@*RESULTS@#There was no statistically significant difference in the latency and amplitude of PRVEPs between right and left eyes. The appearance probability of all the waves was 100% at the different stimulations but that of N1 was 36.77% at the central-field. There was statistically significant difference in the latency of N1 at the full field and peripheral field stimulations and there was no statistically significant difference in the amplitude. There was statistically significant difference in the latency and amplitude of P1 at the full field, central field and peripheral field stimulation.@*CONCLUSION@#The PRVEPs evoked by different field stimulations can be used to evaluate the functions of the different area retina and can be used to identify the exaggerated or pretended visual dysfunction.

Advancement in the study on electrophysiology of color vision and its applicability in forensic medicine / 法医学杂志

Color vision is a major component of vision function. Examination of color vision mostly relies on subjectivity at present. It is important to establish an objective method of color vision examination that would have particular clinical significance and forensic medicine value. With the development of new technology, the examination of visual electrophysiology has become a basic method of clinical diagnosis and investigation. Electroretinogram (ERG) could discriminate different cone cells and color abnormality. Visual evoked potential (VEP) generated characteristic negative wave after stimulation with different colors. Different visual electrophysiology detection methods were reviewed and in particular important new advancement of color vision research of ERG, F-VEP and P-VEP were introduced. The application prospect of color vision research in the clinical forensic medicine was also discussed. It is likely that color vision research study and visual electrophysiology will provide objective evaluation of retina and brain function.

The retrospective analysis of 23 cases of post-traumatic abortion / 法医学杂志

OBJECTIVE@#To analyze the clinical characteristics of post-traumatic abortion and the relationship between trauma and abortion.@*METHODS@#We collected and analyzed 23 cases of post-traumatic abortion encountered in the department of clinical forensic medicine, faculty of forensic medicine, China Medical University in recent years.@*RESULTS@#All 23 post-traumatic abort inns were early abortions. Their traumata were mild and external only. Vagina bleeding and abortion occurred shortly after trauma. Most patients were older, from countryside, or unemployed.@*CONCLUSION@#The causes of abortion are complex. Post-traumatic abortion can only be diagnosed after excluding non-traumatic factors. For those cases abortion occur shortly after injuries and non-traumatic factors can not be excluded, trauma should be assessed for its role in abortions as primary, secondary or induction factors correlated with clinical symptoms and pre-

The application of the same frequency tone masking test in forensic identification / 法医学杂志

OBJECTIVE@#To investigate the relationship between minimum contralateral masking level (MCML) and pure tone threshold of the masking ears by the same frequency tone masking test.@*METHODS@#The pure tone thresholds of 30 subjects (60 ears) were measured by pure tone audiometry and MCML was obtained by means of the same frequency tone masking test.@*RESULTS@#When the stimulus was at the threshold of masked ears, the differences between MCML and pure tone threshold of masking ears were among 0-30 dBHL. 82.4 percent of results showed the differences not higher than 10 dBHL, 97.1 percent of results showed the differences not higher than 15 dBHL. When the stimulus was at 10 dBHL above the threshold of masked ears, the differences between MCML and pure tone threshold of masking ears were among 0-35 dBHL. 90.5 percent of results showed the differences not higher than 25 dBHL, 98.1 percent of results showed the differences not higher than 30 dBHL.@*CONCLUSION@#The real thresholds can be deduced correctly by the same frequency tone masking test.